THE CAUSES OF INTESTINAL ANASTOMOTIC LEAKAGE IN EXPERIMENTAL PERITONITIS

Objective. To determine the leading factor in pathogenesis of intestinal anastomotic failure peritonitis. Methods. The experiment was carried out on non-linear white rats (n=40), divided into 4 equal groups: with peritonitis (group 1), hypovolemia 2), combination 3) and comparison 4). Colonic “end-to-end” anastomosis performed all rats. Peritonitis caused by injection 10% unfiltered feces abdominal cavity. Hypovolemia modeled during operation creating bleeding from branch iliocolic artery.The were taken 3<sup>rd</sup> day after operation, state cavity assessed. histological examination sites performed. content hypoxia biomarkers (HIF-1a, VEGF-C, VEGF-R1) tissue also evaluated ELISA. approved local ethics committee. Results. In group 1, detected characterized a severe general condition due to groups 2 3 statistically significant relationship found between decrease rectal temperature (>2 °C) colonic (p<0.05). Morphological analysis showed most pronounced inflammatory microcirculatory changes 3. Statistically differences level VEGF-C (p=0.0034) VEGF-R1 (p=0.04795) groups. Maximal ischemia zone Conclusion. is considered be as result impairedblood supply depletion mesenteric blood flow. Monitoring successful correction hemodynamic disturbances perioperative period may become prospect for treatment patients requiring under these conditions. What this paper adds For first time determination has been studied.